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Post: The Fallacy of Phase Contrast Microscopy (PCM) Clearance Air Sampling, or 5 Reasons Why We Should Stop Using PCM for Clearance.

The Environmental Protection Agency’s (EPA’s) Asbestos Hazard Emergency Response Act (AHERA) regulation introduced the requirement of clearance sampling after an asbestos abatement project was completed.  Remember the AHERA regulation applies to schools from Kindergarten to 12th grade (K-12), both public and private schools.  However, for all intents and purposes, the AHERA method serves as the industry standard when final clearance is performed for most asbestos abatement projects, especially when areas are to be re-occupied.  The requirements for clearance are found in two sections of the rule:
  • Response Actions; §763.90 (i)
  • Appendix A (to Subpart E) – Interim Transmission Electron Microscopy Analytical Methods – Mandatory and NonMandatory – and Mandatory Section to Determine Completion of Response Actions

AHERA allows final clearance air sampling to be done by phase contrast microscopy (PCM) methodology for projects less than or equal to 160 square feet (SF) or 260 linear feet (LF) by the National Institute for Occupational Safety and Health (NIOSH) 7400 methodology (Issue 3: 14 June 2019 is the current issue).  For projects greater than 160 SF or 260 LF clearance shall be done by the AHERA transmission electron microscopy (TEM) method (requirements at 763.90 (i) (4) and Appendix A).  Since this article’s purpose is to discuss why we should not be using the PCM method we will focus our discussion on this method specifically.  An important point to remember is that the method was designed for personal sampling of workers in environments with actual asbestos exposures.  AHERA adapted the method for clearance requiring that each sample must be less than or equal to a limit of quantitation (LOQ) for PCM of 0.01 fibers per cubic centimeter (f/cc).

Over the years, it has become abundantly clear that the PCM method should not be used for clearance sampling.  The top five reasons it should not be used for clearance sampling are:

Size of the Fibers Analyzed

The rules for the PCM method specifically require the microscopist to count only fibers that are greater than 5 microns long and width greater than or equal to 0.25 microns.  All other fiber lengths and widths are not counted or may not even be seen.  At the Professional Abatement Contractors of New York’s (PACNY’s) 2022 Environmental Conference Lee Poye, Vice President Emeritus Eurofins Built Environment, discussed his presentation “Asbestos in Human Tissue and the Environment – Does Size Matter?” 

  

Lee Poye Presenting at PACNY 2022
 
From Mr. Lee Poye’s presentation, we know that size does matter regarding diseased human tissue.  The type of fibers seen in diseased tissue is less than 5 microns and less than 0.25 microns in width.  So why are we using a method that does not detect the fibers that actually cause disease for clearance?

 

Lee Poye Presenting at PACNY 2022

 

Is the Work Area Actually Clean?

See our Asbestos Floor Tile Debate Results Post and our article in Healthy Buildings.  Our major points were:

  • When using the AHERA method for clearance, what was the typical size of the fibers found?  The answers we got were 58.8% less than 5 microns; 29.4% of both sizes were equal amounts; and 11.8% greater than 5 microns.
  • Have you ever encountered during asbestos flooring removal when utilizing both the NIOSH 7400 (PCM) & the AHERA (TEM) methods of analyses, that the NIOSH 7400 passed while the AHERA method failed?  The answers we got were 52.6% yes, 36.8% no, and 10.5% never used both.
  • AHERA TEM method counts for total asbestos structures per cubic centimeter averaged 22 times greater than the PCM fiber counts on the same filters.
  • AHERA TEM asbestos concentrations obtained during mastic removal with a commercial mastic remover averaged 11 times higher than those measured when removal used amended water.
These points all bring up the question of whether the work area is actually clean when we use the PCM method.

Is 0.01 fibers/cubic centimeter (f/cc) Safe?

Well based on the World Health Organization (WHO), and the EPA there is no safe level of exposure.  If we look at how many asbestos fibers we are breathing in at 0.01 f/cc if we were making a moderate effort it would be approximately 100 asbestos fibers per minute or for an 8-hour day it would be 48,000 asbestos fibers.  If we look at the amount of asbestos fibers in a cubic foot of space it would be 283 asbestos fibers/CF.  So what is the risk at 0.01 f/cc?  In 2021, the Committee for Risk Assessment (RAC) prepared an expert opinion for the European Chemical Agency (ECHA) on the scientific evaluation of occupational exposure limits for asbestos. They have concluded that there is no “safe” level of asbestos exposure. Instead, they provided an exposure-risk relationship to express the excess risk of cancer at different levels of asbestos exposure.  According to the RAC, the risk of excess lifetime cancer risks is 12 cases per 100,000 exposed at 0.01 f/cc. 
 

 

 

Based on this information a better clearance level would be 0.001 f/cc.

 

Are We Sampling Correctly?
 
Before you read this section can you click on the link and answer this question: If you could answer this question?  In our blog post The Reality of Asbestos Clearance Air Sampling! Are You Sampling Enough? and our article in Healthy Buildings “You Can’t Handle The Truth….About Clearance Sampling.”  The major points from these articles were:
  • Many believe or have been misled to believe that PCM sampling is the same as TEM sampling in terms of sampling volume.  This is not the case.  A PCM sample volume meeting AHERA clearance requirements are not at 1200 liters.  To do so is outside of the NIOSH 7400 method requirements for this purpose.  In the NIOSH 7400 method, the issue regarding “relatively clean” environments” is addressed on page 4, number 4, note number 1 which states  “In relatively clean atmospheres, where targeted fiber concentrations are much less than 0.1 f/cc, use larger sample volumes (3000 to 10,000 liters) to achieve quantifiable loadings.”  Even though the formula calculates that 3,850 liters of air should be collected, many people use note 1 to collect 3,000 liters of air for clearance.  Either way clearance samples should be collected using no less than 3,000 liters of air as the minimum allowed for the NIOSH 7400 method requirements and AHERA compliance. 
In New York State the recommended sampling volume is 1,200 liters of air (based on the NYSDOH Environmental Laboratory Approval Program (ELAP) and the Bureau of Occupational Health and the NYSDOL FAQ#13) and in the New York City Department of Environmental Protection (NYCDEP) Title 15 the required sampling volume is 1,800 liters of air for PCM clearance.  Based on the LOQ formula what are the consequences of not collecting the required volume?  Remember this is a formula and if you modify the formula to solve for L instead of t.  You then plug in the amount of time you’re actually sampling for then you get the actual result you are achieving.
 


The NIOSH 7400 method, utilizes the formula above to determine the amount of time needed to achieve the fiber density, E, for optimum filter loading.  So, the minimum density the method allows is 100 fibers per square millimeter (mm2).  The Ac is the collection area for a 25-mm cassette which is 385 mm2.  The Q is the sampling flow rate in LPM, so let’s say that is 16 (the maximum flow rate allowed by the method).  The t is the time we are collecting the sample 1200 liters of air divided by 16 liters per minute gives us 75 minutes.  So if you plug these numbers into the formula you get 0.032 fibers/cubic centimeter is the number you would actually clear at.  For NYC requirements, the flow rate (Q) is a maximum of 15 liters per minute with a volume of 1800 liters for a time of 120 minutes.  Plugging these numbers in means 0.021 f/cc is the actual clearance in NYC.

 
According to the RAC, the risk of excess lifetime cancer risks is 25 cases per 100,000 exposed at 0.02 f/cc and somewhere between 25 and 65 cases per 100,000 exposed at 0.03 f/cc.

The Cost of Clearance Sampling

We know what you are going to say TEM samples cost way more than PCM samples.  We agree they do, but not compared to when AHERA first came out.  When AHERA first came out there were hardly any laboratories that did TEM analysis and those that did the samples cost around $500 per sample.  The difference between PCM analysis costs and TEM analysis costs has come way down.  A recent quote we received from a reputable laboratory for PCM analysis with a 3-hour turnaround was $12.50 per sample while TEM AHERA analysis with a 4-hour turnaround was $150 per sample.  The price difference is smaller than it once was and considering the above points we would say PCM is not worth the money nor the paper it is printed on. 

 

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Future Environment Designs, Inc.

AngeloGarcia.0

Angelo Garcia, III, CIEC, CEOP, Principal-Industrial Hygienist

Welcome to my blog (I really don’t like that word). This is where I will post items of interest and discussions. I am the Principal- Industrial Hygienist (owner, cook, and bottle washer) of Future Environment Designs, Inc. Hope you enjoy this site as much as I do.

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